2018-07-25 07:19:492025-05-21 03:57:10
locus
BSU23520
BSU_23520
geneLength
447
450
product
transcriptional repressor Fur family
transcriptional repressor [SW|Fur family]
outlinks
bsu
BSU23520
BSU_23520
Gene
Phenotypes of a mutant
no growth with glucose and ammonium as single sources of carbon and nitrogen, respectively (due to [[protein|FsrA]]-mediated repression of the ''[[gene|gltA]]-[[gene|gltB]]'' operon) [Pubmed|22389480]
poor frowth on lactate as single carbon source (due to overexpression of [[protein|FsrA]]-mediated repression of the ''[[gene|lutA]]-[[gene|lutB]]-[[gene|lutC]]'' operon, can be suppressed by inactivation of ''[[gene|fsrA]]'' or ''[[gene|fbpB]]'') [Pubmed|22427629]
transcription profile of a ''[[gene|fur]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27416 GEO] [Pubmed|22389480]
no growth with glucose and ammonium as single sources of carbon and nitrogen, respectively (due to [[protein|FsrA]]-mediated repression of the ''[[gene|gltA]]-[[gene|gltB]]'' operon) [Pubmed|22389480]
poor growth on lactate as single carbon source (due to overexpression of [[protein|FsrA]]-mediated repression of the ''[[gene|lutA]]-[[gene|lutB]]-[[gene|lutC]]'' operon, can be suppressed by inactivation of ''[[gene|fsrA]]'' or ''[[gene|fbpB]]'') [Pubmed|22427629]
transcription profile of a ''[[gene|fur]]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27416 GEO] [Pubmed|22389480]
The protein
Protein family
[SW|Fur family]
[SW|Fur family] (according to UniProt)
Biological materials
Mutant
MGNA-C426 (yqkL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2424 NBRP B. subtilis, Japan]
HB6543 (aphA3), available in the [SW|John Helmann] lab; also available in the [SW|Stülke] lab GP879 (''fur::mls'') and GP868 (''fur::mls'', ''perR::spc'')
1A910 ( ''fur''::''kan''), [Pubmed|12029044], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A910&Search=1A910 BGSC]
BKE23520 (Δ[[gene|fur]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCCCTCCTACGC, downstream forward: _UP4_TAGACGGTGCCGAGCGCGAA
BKK23520 (Δ[[gene|fur]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCCCTCCTACGC, downstream forward: _UP4_TAGACGGTGCCGAGCGCGAA
MGNA-C426 (yqkL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2424 NBRP B. subtilis, Japan]
HB6543 (aphA3), available in the [SW|John Helmann]'s lab
GP879 (''fur::mls'') and GP868 (''fur::mls'', ''perR::spc''), available in the [SW|Jörg Stülke]'s lab
1A910 ( ''fur''::''kan''), [Pubmed|12029044], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A910&Search=1A910 BGSC]
BKE23520 (Δ[[gene|fur]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCCCTCCTACGC, downstream forward: _UP4_TAGACGGTGCCGAGCGCGAA
BKK23520 (Δ[[gene|fur]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCCCTCCTACGC, downstream forward: _UP4_TAGACGGTGCCGAGCGCGAA
Labs working on this gene/protein
[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
References
Other original publications
The protein
Structure
[PDB|4ETS] (from Campylobacter jejuni, 33% identity) [pubmed|22665794]
labs
[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]